Action Patterns of Phosphorylase and Glycogen Synthetase on Glycogen
نویسندگان
چکیده
منابع مشابه
Effects of glucose on glycogen synthetase, phosphorylase, and glycogen deposition in the perfused rat liver.
An increase in the perfusate glucose concentration from near zero to about 11 mM increased glycogen synthesis in the perfused, isolated rat liver from zero to a value about half the maximum seen in the intact animal. Increased synthesis appeard to due not only to provision of substrate but also to conversion of glycogen synthetase to the active form and of glycogen phosphorylase to the inactive...
متن کاملComparative Studies on Glycogen Phosphorylase
The isolation and crystallization of both forms of glycogen phosphorylase from human skeletal muscle (1) have made possible a detailed study of the properties of these enzymes in comparison with those of rabbit skeletal muscle (2, 3). Phosphorylase b and a from both species have been shown to crystallize under similar conditions and to have similar pH optima, electrophoretic mobilities, sedimen...
متن کاملLiver Glycogen Phosphorylase Kinase
Liver glycogen phosphorylase kinase was assayed by measuring the radioactivity of [y-32P]ATP incorporated into rabbit skeletal muscle phosphorylase b. Using this assay two forms of phosphorylase kinase, Kinase I and Kinase II, were pirified about 70and 130-fold, respectively, from rabbit liver cytosol by DEAE-Sephadex A50 column chromatography, ammonium sulfate fractionation, gel filtration on ...
متن کاملORDERED MECHANISM OF BINDING OF PHOSPHORYLASE KINASE AND GLYCOGEN PHOSPHORYLASE b TO GLYCOGEN
The kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen has been studied using turbidimetric method (40 mM Hepes, pH 6.8, and 8.2; 20◦C). The binding of phosphorylase kinase with glycogen occurs only in the presence of Ca and Mg . According to the kinetic data, phosphorylase b favors the binding of phosphorylase kinase with glycogen. This conclusion is suppo...
متن کاملGlycogen Phosphorylase in DictyosteLium discoideum
The glycogen phosphorylase of Dictyostelium discoideum has been purified over 200-fold from cells in the culmination stage of development. Analytical gel electrophoresis of the purified enzyme indicates one major protein band with a molecular weight of approximately 210,000. Gel elution verified the presence of phosphorylase activity associated with the protein band. Electrophoresis of partiall...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1970
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1970.tb01109.x